BAICALEIN-RICH FRACTION AS A POTENTIAL RADIOSENSITIZER OR RADIOPROTECTIVE FOR HDR BRACHYTHERAPY: A PRELIMINARY STUDY
Abstract
Abstract
Usage of anti-cancer drugs as radiosensitizers is deemed necessary for the improvement of cancer radiation treatments. In avoiding the negative aftereffects of the drugs, natural compounds are being considered. However, plant compounds are usually highly antioxidative. Baicalein-rich fraction (BRF) which had been found to have an anti-cancer effect, could be a radiosensitizer or radioprotector. In this study, cytotoxicity of BRF was first investigated in MCF-7, MDA-MB-231, and NIH/3T3 cells. Brachytherapy was conducted with dose of 0 to 4 Gy before measuring reactive oxygen species (ROS) production and clonogenic assay. 25% of inhibition concentration by BRF treatment on MCF-7 cell line was found to be the lowest with value of 2.71 µg/ml. Following this concentration, the cells were treated before brachytherapy. The findings show higher post-irradiation ROS induction for BRF treated compared to the control. The highest ROS level was found in MDA-MB-231 cells. However, sensitization enhancement ratios calculated from survival curves show values of 0.96, 0.87. 0.60 for MCF-7, MDA-MB-231, and NIH/3T3 cells, respectively. The amount of ROS generated may be insufficient to damage the cells and was immediately counter-reacted by the BRF-induced antioxidants. Thus, the BRF is inferred to be a radioprotective natural chemical.
AbstraK
Penggunaan ubat-ubatan anti-barah sebagai radiosensitizer dianggap perlu untuk peningkatan rawatan radiasi barah. Untuk mengelakkan kesan negatif ubat-ubatan, sebatian semula jadi dipertimbangkan. Walau bagaimanapun, sebatian tumbuhan biasanya sangat antioksidan. Fraksi kaya Baicalein (BRF) yang didapati mempunyai kesan anti-barah, boleh menjadi radiosensitizer atau radioprotector. Dalam kajian ini, sitotoksisitas BRF pertama kali diselidiki di sel MCF-7, MDA-MB-231, dan NIH / 3T3. Brachytherapy dilakukan dengan dos 0 hingga 4 Gy sebelum mengukur pengeluaran spesies oksigen reaktif (ROS) dan pengujian klonogenik. 25% kepekatan perencatan oleh rawatan BRF pada garis sel MCF-7 didapati paling rendah dengan nilai 2.71 µg / ml. Berikutan kepekatan ini, sel-sel dirawat sebelum brachytherapy. Hasil kajian menunjukkan induksi ROS pasca penyinaran lebih tinggi untuk rawatan BRF berbanding kawalan. Tahap ROS tertinggi dijumpai dalam sel MDA-MB-231. Walau bagaimanapun, nisbah peningkatan pemekaan yang dikira dari keluk kelangsungan hidup menunjukkan nilai 0.96, 0.87. 0.60 untuk sel MCF-7, MDA-MB-231, dan NIH / 3T3, masing-masing. Jumlah ROS yang dihasilkan mungkin tidak mencukupi untuk merosakkan sel dan segera bertindak balas oleh antioksidan yang disebabkan oleh BRF. Oleh itu, BRF disimpulkan sebagai radiopelindungan semula jadi bahan kimia.
