Purification and concentration of gallium-68 via anion exchange method from a SnO2 -based column germanium-68/ gallium-68 generator
Abstract
ABSTRACT
PET/CT Scan with 68Ga-labelled analogs is of increasing interest in Nuclear Medicine and currently is being performed all over the world. However, such labeling procedure requires high purity and concentrated solutions of 68Ga. Here we report the purification and concentration of the eluate of SnO2-Based 68Ge/68Ga generators via the anion exchange method. Three different anion columns were selected to purify and concentrate the68Ga eluates which are Chromafix, Oasis WAX and AG 1-X8 columns. The different anion columns were compared and evaluated in terms of their capability in adsorption and desorption of 68Ga from the generator. While the optimum molarity of Hydrocholric Acid (HCl) for highest 68Ga retention was also determined starting from the ranges of 4M to 7M of HCl. The results showed that the percentage of 68Ga retention or adsorption started to be plateau at molarity of 5.5M for all three anion cartridges. One-way ANOVA analysis proved that there is no significant difference between 5.5M with 6.0M, 6.5M and 7.0M which means that the retention of gallium-68 is equal at those molarities. At 5.5M, Chromafix and Oasis WAX cartridges showed the highest retention of gallium-68 which is 98.30%. The lowest 68Ga retention was gained by AG 1-X8 column which is 97.07%. While for desorption of 68Ga, the highest percentage was obtained by using Oasis WAX cartridges which is 70.49% followed by Chromafix which is 70.36%. The lowest desorption of gallium-68 was obtained by using AG 1-X8 column which is only 58.56%. Therefore, from this study, the most suitable cartridge and HCl molarity that should be applied in purification and concentration of Gallium-68 eluate from a SnO2 Based 68Ge/68Ga generator is Oasis® WAX column with a HCl molarity of 5.5M respectively.
ABSTRAK
PET/CT Scan with 68Ga-labelled analogs is of increasing interest in Nuclear Medicine and currently is being performed all over the world. However, such labeling procedure requires high purity and concentrated solutions of 68Ga. Here we report the purification and concentration of the eluate of SnO2-Based 68Ge/68Ga generators via the anion exchange method. Three different anion columns were selected to purify and concentrate the68Ga eluates which are Chromafix, Oasis WAX and AG 1-X8 columns. The different anion columns were compared and evaluated in terms of their capability in adsorption and desorption of 68Ga from the generator. While the optimum molarity of Hydrocholric Acid (HCl) for highest 68Ga retention was also determined starting from the ranges of 4M to 7M of HCl. The results showed that the percentage of 68Ga retention or adsorption started to be plateau at molarity of 5.5M for all three anion cartridges. One-way ANOVA analysis proved that there is no significant difference between 5.5M with 6.0M, 6.5M and 7.0M which means that the retention of gallium-68 is equal at those molarities. At 5.5M, Chromafix and Oasis WAX cartridges showed the highest retention of gallium-68 which is 98.30%. The lowest 68Ga retention was gained by AG 1-X8 column which is 97.07%. While for desorption of 68Ga, the highest percentage was obtained by using Oasis WAX cartridges which is 70.49% followed by Chromafix which is 70.36%. The lowest desorption of gallium-68 was obtained by using AG 1-X8 column which is only 58.56%. Therefore, from this study, the most suitable cartridge and HCl molarity that should be applied in purification and concentration of Gallium-68 eluate from a SnO2 Based 68Ge/68Ga generator is Oasis® WAX column with a HCl molarity of 5.5M respectively.
